Day 2: 13 Nov 2019 - 10:25 – 11:25 AM
A study was conducted to identify the environmental reservoirs of Enterocytozoon hepatopenaei (EHP) in P. vannamei. The shrimp were collected from three ponds that presented signs of heavy EHP infection with shrimp average daily growth (ADG) lower than 0.1 g/day. The hepatopancreas (HP) of 10 animals per pond were collected as well as plankton (Chaetonotidae / Dreissenidae/ Macrostomidae) specimens that had been trapped on a 20 µm-plankton net for DNA purification. In order to confirm if these zooplankton were potential carriers of EHP, a co-habitation assay was performed to co-culture the EHP infected shrimp with the zooplankton. Histological examination and in situ hybridisation was used to evaluate the mechanical or infectious carrier status of these zooplankton.